Dimethylarginine Asymmetric ADMA 4um vs 2.o Diamond Hydride Column - AppNote
May 29, 2013
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Date: 2--MAY-2013 Last Updated: 21-AUGUST-2026
ADMA can be retained using a HILIC gradient method with the Cogent Diamond Hydride™ HPLC column.Retention times for ADMA differed only slightly between the 4um and 2.o™ stationary phases. Efficiency however was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:
Method Conditions:
Column: Cogent Diamond Hydride
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H2O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
| Time (minutes) | %B |
|---|---|
| 0 | 90 |
| 5 | 30 |
| 8 | 30 |
| 10 | 90 |
For more information: Cogent Diamond Hydride Product Page